Plasmid

Part:BBa_J100377

Designed by: Zachary Shaver   Group: Campbell M Lab   (2018-04-09)


rClone Red with Riboswitch C Mutant #3

This is an rClone Red plasmid (J119384) that replaces the GFP cassette with the riboswitch C (RS-C) mutant #3. This mutant was produced by evolving RS-C, which turns "on" in the presence of theophylline, with phage assisted continuous evolution (PACE) in an attempt to create an RS-C mutant that responds to xanthine instead of theophylline. The RS-C mutant #3 sequence is one of ten RS-C mutants tested for xanthine specificity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 662
    Illegal AgeI site found at 774
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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